Human T-Activator CD3/CD28 Beads, available from Biogradetech under the catalog number C-BETA2001, are specifically designed for the separation and in vitro expansion of human T cells. These beads find applications in various T cell culture technologies, including CAR-T cell therapy. By leveraging the power of anti-human CD3 and anti-human CD28 antibodies conjugated to magnetic beads, Human T-Activator CD3/CD28 Beads provide a simple and effective method for isolating and expanding human T cells.
Product Information
- Catalog: C-BETA2001
- Reactivity: Human
- Concentration: 2 × 108 beads/mL
- Particle size: 4.5 μm
- Endotoxin: 1 EU/mL
- Usage: In vitro T cell activation and expansion in enriched T cell or PBMCs
- Formulation: Phosphate-buffered saline (PBS) containing Human Serum Albumin (HSA), pH 7.4
- Stability: 24 months
Materials Required
- Human T cell culture media.
- Human cytokines for optimal expansion, such as IL-2, IL-7, IL-15.
Protocol
- Wash Human T-Activator CD3/CD28 Beads
- Resuspend the beads in the tube and vortex or tilt and rotate to ensure proper mixing.
- Transfer the desired volume of beads to a new tube.
- Add an equal volume of PBS (containing 1% HSA) for resuspension.
- Place the tube on a magnet for 1 minute and discard the supernatant.
- Remove the tube from the magnet and resuspend the beads in the same volume of PBS (containing 1% HSA).
- Separate CD3+ T cells
- Gently resuspend Ficoll-isolated PBMCs in PBS (containing 1% HSA) and adjust the cell density.
- Add the washed beads to the PBMCs in the appropriate ratio.
- Rotate the samples and incubate at room temperature.
- Dilute the mixture of beads and cells with T cell culture media or PBS (containing 1% HSA) and place the tube on a magnet.
- Remove the supernatant and resuspend the mixture in T cell culture media containing IL-2.
- T cell activation and expansion
- Count the number of T cells daily starting from day 3.
- Dissociate the beads and cells by gently blowing the cell suspension during the later stages of cell expansion.
- When CD3+ T cells exceed 1 × 106 cells/mL, dilute the cells with T cell culture media containing IL-2.
- At the end of the culture (day 9-14), count the cells and remove the beads using magnets.
Product Data
Fig.1 Magnetically separate CD3+ T cells. PMBCs were incubated for 30 min with Human T-Activator CD3/CD28, Beads (Cat. No# C-BETA2001) at a ratio of 3 beads per cell and the positive (isolated) fraction was analyzed isolation efficiency by flow cytometry.
Fig.2 Purified human CD3+T Cells expansion. The purified human CD3+ T cells were stimulated using Human T-Activator CD3/CD28, Beads (Cat. No # C-BETA2001). Cells were expanded in T cell culture medium containing 300 IU/mL of IL-2. Activated Cells were expanded for up to 13 days (A) with high cell viability (B).
Conclusion
Biogradetech’s Human T-Activator CD3/CD28 Beads provide a reliable and efficient solution for the isolation and expansion of human T cells in vitro. By leveraging the power of anti-CD3 and anti-CD28 antibodies conjugated to magnetic beads, these beads enable the activation and expansion of T cells for various applications, including CAR-T cell therapy. Trust Biogradetech for high-quality products that drive advancements in T cell research.
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