| Supplier | BiogradeTech |
| Catalog | BG310285 |
| Size | 96T |
| Storage | 4℃ |
| Background | |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Host | |
| Clone | Monkey |
| Reactivity | Sandwich |
| Apllication |
| Supplier |
| BiogradeTech |
| Catalog |
| BG310285 |
| Size |
| 96T |
| Storage |
| 4℃ |
| Background |
| Description |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Host |
| Clone |
| Monkey |
| Reactivity |
| Sandwich |
| Apllication |
Biogradetech is a leading supplier of essential materials and substances for the global life science and pharmaceutical research and development industry.
Location: 123 Beaver Street, Suite 204，Waltham, MA 02452, USA
E-mail: order@biogradetech.com