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Monkey S100A8(S100 Calcium Binding Protein A8) ELISA Kit

Monkey S100A8(S100 Calcium Binding Protein A8) ELISA Kit

Price: In stock

Description

Supplier BiogradeTech
Catalog BG310155
Size 96T
Storage 4℃
Background
Description The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian S100A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian S100A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian S100A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian S100A8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Host
Clone Monkey
Reactivity Sandwich
Apllication
Supplier
BiogradeTech
Catalog
BG310155
Size
96T
Storage
4℃
Background
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian S100A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian S100A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian S100A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian S100A8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Host
Clone
Monkey
Reactivity
Sandwich
Apllication

Comment

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