Human T-Activator CD3/CD28 Beads, available from Biogradetech under the catalog number C-BETA2001, are specifically designed for the separation and in vitro expansion of human T cells. These beads find applications in various T cell culture technologies, including CAR-T cell therapy. By leveraging the power of anti-human CD3 and anti-human CD28 antibodies conjugated to magnetic beads, Human T-Activator CD3/CD28 Beads provide a simple and effective method for isolating and expanding human T cells.
Product Information
Catalog: C-BETA2001
Reactivity: Human
Concentration: 2 × 108 beads/mL
Particle size: 4.5 μm
Endotoxin: 1 EU/mL
Usage: In vitro T cell activation and expansion in enriched T cell or PBMCs
Formulation: Phosphate-buffered saline (PBS) containing Human Serum Albumin (HSA), pH 7.4
Stability: 24 months
Materials Required
Human T cell culture media.
Human cytokines for optimal expansion, such as IL-2, IL-7, IL-15.
Protocol
Wash Human T-Activator CD3/CD28 Beads
Resuspend the beads in the tube and vortex or tilt and rotate to ensure proper mixing.
Transfer the desired volume of beads to a new tube.
Add an equal volume of PBS (containing 1% HSA) for resuspension.
Place the tube on a magnet for 1 minute and discard the supernatant.
Remove the tube from the magnet and resuspend the beads in the same volume of PBS (containing 1% HSA).
Separate CD3+ T cells
Gently resuspend Ficoll-isolated PBMCs in PBS (containing 1% HSA) and adjust the cell density.
Add the washed beads to the PBMCs in the appropriate ratio.
Rotate the samples and incubate at room temperature.
Dilute the mixture of beads and cells with T cell culture media or PBS (containing 1% HSA) and place the tube on a magnet.
Remove the supernatant and resuspend the mixture in T cell culture media containing IL-2.
T cell activation and expansion
Count the number of T cells daily starting from day 3.
Dissociate the beads and cells by gently blowing the cell suspension during the later stages of cell expansion.
When CD3+ T cells exceed 1 × 106 cells/mL, dilute the cells with T cell culture media containing IL-2.
At the end of the culture (day 9-14), count the cells and remove the beads using magnets.
Product Data
Fig.1 Magnetically separate CD3+ T cells. PMBCs were incubated for 30 min with Human T-Activator CD3/CD28, Beads (Cat. No# C-BETA2001) at a ratio of 3 beads per cell and the positive (isolated) fraction was analyzed isolation efficiency by flow cytometry.
Fig.2 Purified human CD3+T Cells expansion. The purified human CD3+ T cells were stimulated using Human T-Activator CD3/CD28, Beads (Cat. No # C-BETA2001). Cells were expanded in T cell culture medium containing 300 IU/mL of IL-2. Activated Cells were expanded for up to 13 days (A) with high cell viability (B).
Conclusion
Biogradetech’s Human T-Activator CD3/CD28 Beads provide a reliable and efficient solution for the isolation and expansion of human T cells in vitro. By leveraging the power of anti-CD3 and anti-CD28 antibodies conjugated to magnetic beads, these beads enable the activation and expansion of T cells for various applications, including CAR-T cell therapy. Trust Biogradetech for high-quality products that drive advancements in T cell research.
For more information or to place an order, please visit our website or contact our sales team.
Human T-Activator CD3/CD28 Beads, available from Biogradetech under the catalog number C-BETA2001, are specifically designed for the separation and in vitro expansion of human T cells. These beads find applications in various T cell culture technologies, including CAR-T cell therapy. By leveraging the power of anti-human CD3 and anti-human CD28 antibodies conjugated to magnetic beads, Human T-Activator CD3/CD28 Beads provide a simple and effective method for isolating and expanding human T cells.
Product Information
Catalog: C-BETA2001
Reactivity: Human
Concentration: 2 × 108 beads/mL
Particle size: 4.5 μm
Endotoxin: 1 EU/mL
Usage: In vitro T cell activation and expansion in enriched T cell or PBMCs
Formulation: Phosphate-buffered saline (PBS) containing Human Serum Albumin (HSA), pH 7.4
Stability: 24 months
Materials Required
Human T cell culture media.
Human cytokines for optimal expansion, such as IL-2, IL-7, IL-15.
Protocol
Wash Human T-Activator CD3/CD28 Beads
Resuspend the beads in the tube and vortex or tilt and rotate to ensure proper mixing.
Transfer the desired volume of beads to a new tube.
Add an equal volume of PBS (containing 1% HSA) for resuspension.
Place the tube on a magnet for 1 minute and discard the supernatant.
Remove the tube from the magnet and resuspend the beads in the same volume of PBS (containing 1% HSA).
Separate CD3+ T cells
Gently resuspend Ficoll-isolated PBMCs in PBS (containing 1% HSA) and adjust the cell density.
Add the washed beads to the PBMCs in the appropriate ratio.
Rotate the samples and incubate at room temperature.
Dilute the mixture of beads and cells with T cell culture media or PBS (containing 1% HSA) and place the tube on a magnet.
Remove the supernatant and resuspend the mixture in T cell culture media containing IL-2.
T cell activation and expansion
Count the number of T cells daily starting from day 3.
Dissociate the beads and cells by gently blowing the cell suspension during the later stages of cell expansion.
When CD3+ T cells exceed 1 × 106 cells/mL, dilute the cells with T cell culture media containing IL-2.
At the end of the culture (day 9-14), count the cells and remove the beads using magnets.
Product Data
Fig.1 Magnetically separate CD3+ T cells. PMBCs were incubated for 30 min with Human T-Activator CD3/CD28, Beads (Cat. No# C-BETA2001) at a ratio of 3 beads per cell and the positive (isolated) fraction was analyzed isolation efficiency by flow cytometry.
Fig.2 Purified human CD3+T Cells expansion. The purified human CD3+ T cells were stimulated using Human T-Activator CD3/CD28, Beads (Cat. No # C-BETA2001). Cells were expanded in T cell culture medium containing 300 IU/mL of IL-2. Activated Cells were expanded for up to 13 days (A) with high cell viability (B).
Conclusion
Biogradetech’s Human T-Activator CD3/CD28 Beads provide a reliable and efficient solution for the isolation and expansion of human T cells in vitro. By leveraging the power of anti-CD3 and anti-CD28 antibodies conjugated to magnetic beads, these beads enable the activation and expansion of T cells for various applications, including CAR-T cell therapy. Trust Biogradetech for high-quality products that drive advancements in T cell research.
For more information or to place an order, please visit our website or contact our sales team.
Biogradetech is a leading supplier of essential materials and substances for the global life science and pharmaceutical research and development industry.
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E-mail: order@biogradetech.com